Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity

1. Preissl, S., Gaulton, K. J. & Ren, B. Characterizing cis-regulatory elements using single-cell epigenomics. Nat. Rev. Genet. https://doi.org/10.1038/s41576-022-00509-1 (2022). This review article presents an overview of single-cell epigenomic technologies.

   Article Google Scholar

2. Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Commmon. 101930 (2019). This paper introduced the protein A–Tn5 transposase fusion protein for single-cell and low-input bulk profiling of histone modifications.

   Article Google Scholar

3. Bartosovic, M., Kabbe, M. & Castelo-Branco, G. Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues. Nat. Biotechnol. 39825–835 (2021). This paper introduced the single-cell profiling of histone modifications on a droplet microfluidic platform.

   Article CAS Google Scholar

4. Stuart, T. et al. Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolution. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01588-5 (2022). This paper, together with our study, introduces nanobody–Tn5 transposase fusion proteins for single-cell and multimodal profiling of histone modifications.

   Article Google Scholar

5. La Manno, G. et al. RNA velocity of single cells. Nature 560494–498 (2018). This paper introduces the concept of RNA velocity of single cells: the ratio of spliced and unspliced mRNA is used to estimate the trajectory and velocity of differentiation.

   Article Google Scholar

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This is a summary of: Bartosovic, M. & Castelo-Branco, G. Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01535-4 (2022).