The study makes major advances in the use of synthetic cells, or protocells, to more precisely mimic the intricate composition, structure, and function of living cells.
The study uses bacteria to bring scientists closer to building these artificial lifelike cells.
Researchers have used bacteria to help develop advanced synthetic cells that imitate the real-life functionality of cells.
The study, conducted by the University of Bristol and published in the journal Nature, advances the development of synthetic cells, or protocells, to more precisely replicate the complex composition, structure, and function of living cells.
Establishing true-to-life functionality in protocells is a global great challenge involving several fields, from the origin of life research to bottom-up synthetic biology and bioengineering. Due to previous failures in modeling protocells using microcapsules, the research team turned to bacteria to construct sophisticated synthetic cells utilizing a living material assembly process.
Professor Stephen Mann from the School of Chemistry at the University of Bristol and the Max Planck Bristol Centre for Minimal Biology, and colleagues Drs. Can Xu, Nicolas Martin (now at the University of Bordeaux), and Mei Li from the Bristol Centre for Protolife Research have demonstrated a method for building highly complex protocells using viscous micro-droplets filled with living bacteria as a microscopic building site.
The group initially exposed the empty droplets to two different types of bacteria. One population was captured spontaneously inside the droplets, while the other was confined at the droplet surface.
Then, both types of bacteria were destroyed so that the released cellular components remained trapped inside or on the surface of the droplets to produce membrane-coated bacteriogenic protocells containing thousands of biological molecules, parts, and machinery.
The researchers discovered that the protocells were able to produce energy-rich molecules (ATP) via glycolysis and synthesize RNA and proteins by in vitro gene expression, indicating that the inherited bacterial components remained active in the synthetic cells.
Further testing the capacity of this technique, the team employed a series of chemical steps to remodel the bacteriogenic protocells structurally and morphologically. The released bacterial DNA was condensed into a single nucleus-like structure, and the droplet interior infiltrated with a cytoskeletal-like network of protein filaments and membrane-bounded water vacuoles.
As a step towards the construction of a synthetic/living cell entity, the researchers implanted living bacteria into the protocells to generate self-sustainable ATP production and long-term energization for glycolysis, gene expression, and cytoskeletal assembly. Curiously, the protoliving constructs adopted an amoeba-like external morphology due to on-site bacterial metabolism and growth to produce a cellular bionic system with integrated life-like properties.
Corresponding author Professor Stephen Mann said: “Achieving high organizational and functional complexity in synthetic cells is difficult, especially under close-to-equilibrium conditions. Hopefully, our current bacteriogenic approach will help to increase the complexity of current protocell models, facilitate the integration of myriad biological components and enable the development of energized cytomimetic systems.”
First author Dr. Can Xu, a Research Associate at the University of Bristol, added: “Our living-material assembly approach provides an opportunity for the bottom-up construction of symbiotic living/synthetic cell constructs. For example, using engineered bacteria it should be possible to fabricate complex modules for development in diagnostic and therapeutic areas of synthetic biology as well as in biomanufacturing and biotechnology in general.”
Reference: “Living material assembly of bacteriogenic protocells” by Can Xu, Nicolas Martin, Mei Li, and Stephen Mann, 14 September 2022, Nature.
DOI: 10.1038/s41586-022-05223-w
Note: This article have been indexed to our site. We do not claim legitimacy, ownership or copyright of any of the content above. To see the article at original source Click Here
Dell OptiPlex 5040 SFF Desktop Computer PC Bundle Setup with New 23.8" FHD Monitor Inter Core i5-6600 3.3GHz 4-Cores 8GB 256GB SSD, Keyboard & Mouse, Wi-Fi, Bluetooth, Windows 10 Pro (Renewed)
$184.99 (as of June 25, 2024 21:05 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
Wire rack cover by Crafted Palm for removable wood shelf covering in closet, kitchen pantry, bathroom, office, hallway or mudroom shelving board upgrade (16 Inch Length, 12 inch deep, Early American)
$63.99 (as of June 24, 2024 21:06 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
USB C Car Charger, 3-Port 67W Dual USB-C & USB-A Car Power Adapter PD/QC Fast Charging Cigarette Lighter for iPhone 15/14/13/12/11/Pro Max, iPad, Samsung Galaxy S24/S23/S22/S21, Google Pixel, Android
$9.99 (as of June 25, 2024 21:05 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
Hearth and Homestead: Handmade "Blue Outback" Australian Whipped Tallow Balm with Emu, Jojoba, Sandalwood, and Blue Cypress - 1.3 oz -
$47.00 (as of June 24, 2024 21:06 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
