- Research Briefing
- Published:
Nature Biotechnology (2023)Cite this article
904 Accesses
2 Altmetric
Subjects
TnpB proteins are hypercompact RNA-guided DNA endonucleases. By systematically mining and characterizing TnpB proteins from the IS605 family, we identified potent genome editors and established a framework for high-throughput annotation and screening of TnpB systems in prokaryotic genomes.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
24,99 € / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
195,33 € per year
only 16,28 € per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Altae-Tran, H. et al. The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Science 37457–65 (2021). This paper characterizes IscB and TnpB, demonstrates IscB’s activity in human cells and reconstructs the evolutionary relationships among IscB, TnpB and Cas proteins.
Karvelis, T. et al. Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature 599692–696 (2021). This paper reports TnpB’s cleavage activity in human cells and proposes the expansion mechanism of IS200/IS605.
Nakagawa, R. et al. Cryo-EM structure of the transposon-associated TnpB enzyme. Nature 616390–397 (2023). This paper reports the cryo-electron microscopy structure of the ISDra2 TnpB–reRNA–dsDNA complex.
Sasnauskas, G. et al. TnpB structure reveals minimal functional core of Cas12 nuclease family. Nature 616384–389 (2023). This paper reports the structure of the ISDra2 TnpB–reRNA–dsDNA complex in relation to the Cas12 family.
Dobzhansky, T. Nothing in biology makes sense except in the light of evolution. Am. Biol. Teach. 35125–129 (1973). In this essay, Dobzhansky introduces the title precept.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Xiang, G. et al. Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01857-x (2023).
Rights and permissions
About this article
Cite this article
High-throughput screening of TnpB proteins identifies hypercompact genome editors. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01858-w
Published:
DOI: https://doi.org/10.1038/s41587-023-01858-w
Note: This article have been indexed to our site. We do not claim legitimacy, ownership or copyright of any of the content above. To see the article at original source Click Here
Ailun 3 Pack Screen Protector for iPhone 15 Pro Max [6.7 inch] + 3 Pack Camera Lens Protector with Installation…
$6.98 (as of July 6, 2024 21:17 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
Nippies Nipple Cover - Sticky Adhesive Silicone Nipple Pasties - Reusable Pasty Nipple Covers for Women with Travel…
$26.50 (as of July 6, 2024 21:17 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
Ring Pop Bulk Candy Lollipop Variety Party Pack -20 Count Lollipops w/ Assorted Flavors - Fun Candy For Birthdays, Party Favors, Pool Parties, 4th of July & Summer Fun - Summer Treats Loved by Kids
$7.70 (as of July 6, 2024 21:18 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
DoHonest Baby Car Camera for Backseat: HD 1080P Easy Setup Carseat Camera Rear Facing Infant - Crystal Night Vision 360° Rotating Baby Car Monitor for Kids - V33
$18.99 (as of July 6, 2024 21:18 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
