- Matters Arising
- Published:
(2023)Cite this article
3581 Accesses
1 Citations
34 Altmetric
Subjects
arising from M. Niu et al. Nature Biotechnology https://doi.org/10.1038/s41587-022-01635-1 (2023)
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
24,99 € / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
195,33 € per year
only 16,28 € per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Niu, M. et al. Droplet-based transcriptome profiling of individual synapses. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01635-1 (2023).
Duve, C. Exploring cells with a centrifuge. Science 189, 186–194 (1975).
Gray, E. G. & Whittaker, V. P. The isolation of nerve endings from brain. J. Anat. 96, 79–88.8 (1962).
Hafner, A.-S., Donlin-Asp, P. G., Leitch, B., Herzog, E. & Schuman, E. M. Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments. Science 364, eaau3644 (2019).
Zhu, F. et al. Architecture of the mouse brain synaptome. Neuron 99, 781–799 (2018).
Dodd, P. R. et al. Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain. Neurochem. Pathol. 4, 177–198 (1986).
Dunkley, P. R. & Robinson, P. J. Synaptosome preparations: which procedure should I use? In Synaptosomes 27–53 (ed. Murphy, K. M.) (Springer, 2018).
Villasana, L. E., Klann, E. & Tejada-Simon, M. V. Rapid isolation of synaptoneurosomes and postsynaptic densities from adult mouse hippocampus. J. Neurosci. Methods 158, 30–36 (2006).
Biesemann, C. Development of Fluorescence Activated Synaptosome Sorting (FASS) and Analysis of VGLUT1 Synapses from Mouse Brain. PhD thesis, Georg August University Göttingen (2010).
Biesemann, C. et al. Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting. EMBO J. 33, 157–170 (2014).
Hobson, B. D. & Sims, P. A. Critical analysis of particle detection artifacts in synaptosome flow cytometry. eNeuro 6, ENEURO.0009-19.2019 (2019).
Hobson, B. D. et al. Subcellular and regional localization of mRNA translation in midbrain dopamine neurons. Cell Rep. 38, 110208 (2022).
Raiteri, M., Sala, R., Fassio, A., Rossetto, O. & Bonanno, G. Entrapping of impermeant probes of different size into nonpermeabilized synaptosomes as a method to study presynaptic mechanisms. J. Neurochem. 74, 423–431 (2000).
Li, X. G., Somogyi, P., Ylinen, A. & Buzsáki, G. The hippocampal CA3 network: an in vivo intracellular labeling study. J. Comp. Neurol. 339, 181–208 (1994).
Shapiro, E., Biezuner, T. & Linnarsson, S. Single-cell sequencing-based technologies will revolutionize whole-organism science. Nat. Rev. Genet. 14, 618–630 (2013).
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Biotechnology thanks the anonymous reviewers for their contribution to the peer review of this work.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Hobson, B.D., Herzog, E. Methodological concerns and lack of evidence for single-synapse RNA-seq.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01877-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41587-023-01877-7
This article is cited by
Note: This article have been indexed to our site. We do not claim legitimacy, ownership or copyright of any of the content above. To see the article at original source Click Here
Amazon Fire TV Stick 4K (newest model) with AI-powered Fire TV Search, Wi-Fi 6, stream over 1.5 million movies and shows, free & live TV
$21.99 (as of November 21, 2024 19:21 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
Nigrin Nano Sparkle Cloth Car Scratch Remover, Nanosparkle Cloths for Car Scratches, Shine Cloths for Car Scratches, with Scratch Repair and Polishing Function
$9.00 (as of November 21, 2024 18:55 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
We Who Wrestle with God: Perceptions of the Divine
$23.89 (as of November 21, 2024 18:55 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
The Housemaid's Wedding: A Short Story
$3.99 (as of November 21, 2024 18:55 GMT +00:00 - More infoProduct prices and availability are accurate as of the date/time indicated and are subject to change. Any price and availability information displayed on [relevant Amazon Site(s), as applicable] at the time of purchase will apply to the purchase of this product.)
