Tagging peptides with DNA allows repeated reads via a helicase in a nanopore with reduced error rates.
Barcode scanners, fingerprint readers and facial recognition technology are a daily part of our lives, providing an easy way to quickly detect and identify various objects, including ourselves. However, as objects become smaller, the task of recognizing them requires tools that are increasingly sophisticated and specialized. For individual biomolecules, nanopores have emerged as a powerful sensor platform, and nanopore sequencing of individual nucleic acids has become a mainstay of biomedical research. In Science, Brinkerhoff et al.1 now report the latest advances in the quest to extend nanopore sequencing to proteins. The authors developed nanopore-based scanners of single-molecule peptide chains that substantially lower the error rate in amino acid identification by obtaining numerous independent reads of the same molecule, bringing the era of single-molecule proteomics one step closer. A massively scalable proof-of-concept peptide readout device can scan through protein fragments, or full-length proteins, and identify the proteome composition in a manner that outputs actionable information about a sample of interest.
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Wanunu, M. Back and forth with nanopore peptide sequencing.
Nat Biotechnol (2022). https://doi.org/10.1038/s41587-021-01205-x
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DOI: https://doi.org/10.1038/s41587-021-01205-x
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